Besides the motion artifacts, collecting such a 3D time lapse stack of 20–30 slices for each time point results in up to thousands of radiation exposures to each cell. The endosome of interest may then travel while the Z-stack is being captured, leaving visible motion artifacts making it difficult to conduct subsequent quantification analysis. During maturation, the endosome, driven by intracellular motors along the cytoskeleton, also moves at various speeds up to a few microns per second in different directions inside the 3D cytoplasmic space ( Kerr and Teasdale, 2014 Scott et al., 2014). This step in the endosome maturation process occurs within a few minutes and involves a dramatic kinetic change of membrane domains ( Bergeland et al., 2008 Skjeldal et al., 2012). This article has an associated First Person interview with the first author of the paper.Ī key event that marks endosome maturation from early to late endosomes, is the change of the Rab proteins coating the endosome, from Rab5 to Rab7 ( Rink et al., 2005). Our calibration images show that the approach provides highly repeatable and stable imaging conditions that enable photometric measurements of the acquired data, in both standard live imaging and super-resolution modes. The device generating the signals has been tested on spinning disk confocal and instant structured-illumination-microscopy (iSIM) microscopes. The implementation minimally requires two synchronized control signals that drive a piezo stage and trigger the camera exposure. With faster imaging, radiation exposure to the sample is reduced, resulting in less fluorophore photobleaching and potential photodamage. By sacrificing detailed information in the Z-direction, we propose a new imaging modality that involves capturing fast ‘projections’ from the field of depth and shortens imaging time by approximately an order of magnitude as compared to standard volumetric confocal imaging. Depending on the shortfall, this may result in undersampling and/or motion artifacts that ultimately limit the quality of the imaging data. I’m more of an ‘80s fan rather than ‘90s because the ‘90s was having an identity crisis and didn’t really know who it was,” said Haslar.Ĭheck out C.M.Despite progress made in confocal microscopy, even fast systems still have insufficient temporal resolution for detailed live-cell volume imaging, such as tracking rapid movement of membrane vesicles in three-dimensional space. The theme is fun though because it’s not like high school dressing up was involved and that was pretty cool. “The theme of the dance was good the DJ just didn’t play any songs from that era so that kind of defeats the purpose of the theme. To some people the theme was a great idea but thought there could have been a change in music. I’m not a big fan of any of the music played in either of those decades, it was bad hair, bad clothes, but good times.” “If I had to vote for the best era of the two, I would probably choose the ‘80s because of Ronald Regan. “I don’t like the theme of the dance because I’m more of a ‘60s and ‘70s kind of guy,” said an anonymous source who is a junior at CSU-Pueblo majoring in Math. Some passerbys also didn’t like the idea of ‘80s vs. “Both decades were very awkward and I can relate to that.” Plus I’m not a big fan of ‘80s music and very little from the ‘90s,”said Josh Cozadd a sophomore double majoring in English and Music. “I’m not really sure I like the theme of the dance because these were the most awkward decades of all time. Some attendees of the dance who didn’t show up for the dancing, had a different opinion about the theme. “Mocktails, that’s what draws me to these activities,” said Matt Haslar, a senior Psychology major at CSU-Pueblo. Many people showed up dressed up to fit the theme, but a few didn’t come to the dance for the theme or the dancing. “A ton of time and paperwork was put into this dance so hopefully there’s a good turn out.” “The theme of the dance was voted on by everyone who was on the Special Events Committee,” said Jenna Rossow a member of the Special Events Committee and a member of the Alpha Sigma Alpha Sorority. Providing the music for the dance was DJ Mike Torres and live ‘80s and ‘90s band Lady’s Night. ‘90s dance held in the OUC Ballroom by the Office of Student Activities staff and the Special Events Committee. Friday January 16 was a night full of “awesome” music and dancing at the ‘80s vs.
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